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Biofilms Quiz
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At insertion of an indwelling medical device, which measure most directly reduces initial bacterial contamination that could seed a biofilm?
- A Using bactericidal agents that target rapidly dividing bacteria You picked ✕
- B Enhancing the host immune response with immunomodulatory drugs You picked ✕
- C Increasing the antibiotic dose to overcome poor penetration You picked ✕
- D Use sterile equipment and aseptic insertion technique You picked ✓
- E Routine long-term broad-spectrum antibiotics given after insertion You picked ✕
Correct!
Incorrect
The answer is D: Use sterile equipment and aseptic insertion technique
Explanation
Why D is correct
See Management. Option D is correct because the most direct way to prevent a biofilm on a device is to stop bacteria reaching it, which means sterile equipment and aseptic technique at insertion. An indwelling device is any device left inside the body, such as a urinary catheter, an intravenous line or a prosthetic joint. A biofilm starts when a few bacteria attach to the surface of the device, so preventing that first contamination prevents the problem. Aseptic technique means steps such as hand hygiene, sterile gloves and a no-touch method, so that bacteria from the skin or the environment do not reach the device. Prevention matters so much because, once a biofilm forms, the bacteria are embedded in a protective matrix, slow their metabolism and divide less, so they are much less susceptible to antibiotics and the immune system. Biofilm infections are mostly iatrogenic, meaning they are caused by medical care, which is why the management of biofilms mainly focuses on prevention. Memory aid: it is far easier to keep bacteria off a device than to remove them once they have built a biofilm.
- Best prevention: sterile equipment and aseptic technique at insertion stop the first bacteria reaching the device.
- Aseptic technique: hand hygiene, sterile gloves and a no-touch method.
- Why prevention matters: an established biofilm is much less susceptible to antibiotics and the immune system.
Why the other options are incorrect
- A. Using bactericidal agents that target rapidly dividing bacteria: Bacteria in a biofilm slow down and divide little, so drugs that depend on rapid division work poorly. This is a treatment idea, not a prevention step at insertion. Your answer
- B. Enhancing the host immune response with immunomodulatory drugs: Biofilms are built partly to resist immune attack, so boosting immunity alone is not enough. It does not stop bacteria reaching the device during insertion. Your answer
- C. Increasing the antibiotic dose to overcome poor penetration: Raising the dose does not reliably clear an established biofilm and increases the risk of toxicity. It also comes too late, because contamination at insertion has already happened. Your answer
- E. Routine long-term broad-spectrum antibiotics given after insertion: Long-term broad-spectrum antibiotics do not reliably stop a biofilm forming and add to the risk of side effects and resistance. They are not the most direct way to prevent contamination. Your answer
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More questions from this set. Start the quiz to answer them and see each explanation.
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1
Which listed organism is classically associated with biofilm infection on prosthetic heart valves and joint replacements?
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2
A 28-year-old woman develops a persistent urinary tract infection after prolonged use of an indwelling urinary catheter. Cultures grow bacteria that are difficult to eradicate with antibiotics despite in-vitro sensitivity. Which biological process best explains the coordinated change in bacterial behaviour that contributes to this antibiotic tolerance?
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